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Table 3 Body condition and immune parameters. Mixed linear models or generalized linear model analysing the factors influencing body condition (a), PO activity (b), Total PO activity (c), haemocyte count (d), the proportion of beetles exhibiting antibacterial activity in their haemolymph (e) and the intensity of this antibacterial activity as the size of the zone of inhibition (f) in both females (left) and males (right). Models included sex-ratio condition, Age in weeks (ordinal variable), their interaction, body condition, and replicates as a random factor

From: Sex-specific patterns of senescence in artificial insect populations varying in sex-ratio to manipulate reproductive effort

 

Females

Males

d.f.

d.f. den.

F or χ2

P

d.f.

d.f. den.

F or χ2

P

A Body condition#

 Age

3

207.9

17.14

< 0.001

3

215.1

6.64

< 0.001

 Sex-ratio

2

98.2

0.95

0.39

2

89.7

0.57

0.565

 Age x Sex-ratio

6

207.6

0.72

0.637

6

215.1

0.23

0.964

B PO activity#

 Age

3

199.8

6.76

< 0.001

3

211.9

8.20

< 0.001

 Sex-ratio

2

86.9

0.14

0.869

2

103.3

3.69

0.028

 Age x Sex-ratio

6

198.3

0.75

0.606

6

211.3

2.21

0.043

 Body condition

1

208.9

6.82

< 0.001

1

222.9

2.25

0.135

C Total-PO activity#

 Age

3

189

10.90

< 0.001

3

201.8

2.49

0.061

 Sex-ratio

2

189

0.21

0.807

2

65.7

1.91

0.155

 Age x Sex-ratio

6

189

0.40

0.877

6

201.2

1.25

0.282

 Body condition

1

189

6.10

0.014

1

210.8

0.83

0.364

D Haemocyte#

 Age

3

193.8

1.28

0.281

3

208

6.90

< 0.001

 Sex ratio

2

11.4

0.04

0.963

    

E Antibacterial activity (proportion)§

 Age

3

 

42.89

< 0.001

3

 

12.879

< 0.001

 Body condition

1

 

1.1902

0.275

1

 

10.595

0.157

 Age

    

3

 

14.391

0.002

 Sex-ratio

    

2

 

2.2331

0.327

F Antibacterial activity (intensity)#1

 Age

3

 

2.27

0.088

    

 Sex-ratio

    

2

 

4.27

0.019

  1. The models presented here are those minimizing the AICc, with ΔAICc > 2. Values where p ≤ 0.05 are given in bold. # Linear model, § GLM, distribution: binomial, link function: Logit, 1 because a low number of animals showed antimicrobial activity in some conditions (Fig. 4b, d), data were not available for some replicates, therefore replicates were omitted for these models