Fig. 6

Chromosome 1 of T. pennellii is a fusion product of three ancestral chromosomes. a Positive control: Hybridization of the T. pennellii painting probe to T. pennellii chromosomes (n = 16). b and c Hybridization of the T. pennellii probe to chromosomes from T. hansoni (Tha, n = 23) and from D. mawsoni (n = 24), respectively. Each panel shows a DAPI-stained karyotype at the top, and one (b, c) or more (a) karyotypes after hybridization of the painting probe. Bound painting probe was imaged by FISH, and detected using fluorescein (green signals). Numbered chromosome pairs are referenced in the text. Scale bars: 10 μm