Table 2 Characterization of the ITS2 molecules of astome ciliates
Taxon | Total length | GC cont-ent | Length of helix | Number of unpaired bases in | No. of bulges in helix III | No. of GU pairings | ΔG (37 °C, kcal/mol) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
I | E1 | II | III | IV | Common loop(s) | Terminal loop of helix II | Terminal loop of helix III | Terminal loop of helix IV | Bulge(s) of helix II | Bulges of helix III | Bulges of helix IV | ||||||
Almophrya bivacuolata | 182 | 46.15 | – | 10 | 26 | 75 | 26 | 45 | 4 | 3 | 11 | 4 | 18 | 1 | 5 | 4 | −48.10 |
Anoplophrya lumbrici | 191 | 48.17 | – | 10 | 26 | 80 | 24 | 51 | 4 | 5 | 11 | 4 | 19 | 1 | 5 | 3 | −48.30 |
Subanoplophrya nodulata | 194 | 37.63 | – | 8 | 28 | 81 | 17 | 60 | 4 | 3 | 11 | 4 | 18 | – | 5 | 4 | −41.10 |
Anoplophrya vulgaris | 184 | 53.80 | – | 10 | 24 | 78 | 19 | 53 | 4 | 5 | 11 | 4 | 19 | – | 5 | 5 | −42.80 |
Eudrilophrya complanata | 186 | 43.55 | – | 6 | 26 | 73 | 19 | 62 | 4 | 5 | 11 | 4 | 26 | – | 5 | 3 | −32.10 |
Metaracoelophrya sp. | 185 | 36.76 | – | 10 | 26 | 74 | 17 | 58 | 4 | 3 | 11 | 4 | 21 | – | 5 | 3 | −35.90 |
Metaradiophrya lumbrici | 188 | 44.68 | 19 | – | 24 | 89 | 29 | 27 | 4 | 7 | 4 | 4 | 18 | 7 | 6 | 8 | −52.30 |
Metaradiophrya varians | 193 | 44.04 | 25 | – | 29 | 89 | 29 | 21 | 5 | 7 | 4 | 4 | 18 | 7 | 6 | 9 | −49.70 |
Njinella prolifera | 188 | 50.53 | – | 6 | 26 | 77 | 17 | 62 | 4 | 3 | 11 | 6 | 24 | – | 5 | 3 | −35.70 |
Paraclausilocola constricta | 183 | 47.54 | – | 6 | 25 | 74 | 19 | 59 | 3 | 4 | 11 | 4 | 20 | – | 5 | 3 | −37.50 |
Minimum | 182 | 36.76 | 19 | 6 | 24 | 73 | 17 | 21 | 3 | 3 | 4 | 4 | 18 | – | 5 | 3 | −52.30 |
Maximum | 194 | 53.80 | 25 | 10 | 29 | 89 | 29 | 62 | 5 | 7 | 11 | 6 | 26 | – | 6 | 9 | −32.10 |
Arithmetic mean | 187.4 | 45.30 | – | – | 26.0 | 79.0 | 21.6 | 49.8 | – | – | – | – | 20.1 | – | – | 4.5 | −42.35 |
Standard deviation | 4.17 | 5.27 | – | – | 1.56 | 5.89 | 4.93 | 14.67 | – | – | – | – | 2.81 | – | – | 2.22 | 6.97 |