Strong differences in the clonal variation of two Daphnia species from mountain lakes affected by overwintering strategy

Background The population structure of cyclical parthenogens such as water fleas is strongly influenced by the frequency of alternations between sexual and asexual (parthenogenetic) reproduction, which may differ among populations and species. We studied genetic variation within six populations of two closely related species of water fleas of the genus Daphnia (Crustacea, Cladocera). D. galeata and D. longispina both occur in lakes in the Tatra Mountains (Central Europe), but their populations show distinct life history strategies in that region. In three studied lakes inhabited by D. galeata, daphnids overwinter under the ice as adult females. In contrast, in lakes inhabited by D. longispina, populations apparently disappear from the water column and overwinter as dormant eggs in lake sediments. We investigated to what extent these different strategies lead to differences in the clonal composition of late summer populations. Results Analysis of genetic variation at nine microsatellite loci revealed that clonal richness (expressed as the proportion of different multilocus genotypes, MLGs, in the whole analysed sample) consistently differed between the two studied species. In the three D. longispina populations, very high clonal richness was found (MLG/N ranging from 0.97 to 1.00), whereas in D. galeata it was much lower (0.05 to 0.50). The dominant MLGs in all D. galeata populations were heterozygous at five or more loci, suggesting that such individuals all represented the same clonal lineages rather than insufficiently resolved groups of different clones. Conclusions The low clonal diversities and significant deviations from Hardy-Weinberg equilibrium in D. galeata populations were likely a consequence of strong clonal erosion over extended periods of time (several years or even decades) and the limited influence of sexual reproduction. Our data reveal that populations of closely related Daphnia species living in relatively similar habitats (permanent, oligotrophic mountain lakes) within the same region may show strikingly different genetic structures, which most likely depend on their reproductive strategy during unfavourable periods. We assume that similar impacts of life history on population structures are also relevant for other cyclical parthenogen groups. In extreme cases, prolonged clonal erosion may result in the dominance of a single clone within a population, which might limit its microevolutionary potential if selection pressures suddenly change.


Background
Many organisms are capable of alternating sexual and asexual reproduction throughout their life cycle, including protists, cnidarians, rotifers, crustaceans, insects, mosses, vascular plants and macro-algae [1]. The relative importance of both reproduction strategies has profound influences on the genetic structure of populations and on the evolutionary response to selection pressures [2]. In many cases, sexual reproduction in such taxa is associated with the formation of dormant stages that ensure population survival during unfavourable periods (i.e., dispersal through time), as well as spatial dispersal [3].
In aquatic environments that undergo regular seasonal changes, including cold temperate lakes or temporary ponds, many zooplankton groups such as water fleas (Cladocera) usually survive unfavourable periods of winter or drought as dormant stages, and hatch at the onset of more favourable conditions. The typical cladoceran life cycle (reviewed in [2]) is thus cyclical parthenogenesis: females reproduce clonally under favourable conditions, and switch to male production and sexual reproduction when conditions deteriorate.
Fertilized dormant eggs, usually encased in an ephippium, enter dormancy at an early embryonic stage, endure unfavourable periods, and can hatch when the conditions improve again. Each hatchling from a dormant egg of a cyclically parthenogenetic cladoceran has a unique genotype [4,5]. Under this scenario, a high number of genotypes may be expected at the beginning of the growing season in such populations. However, due to selection and drift during the prolonged period of parthenogenetic reproduction, some clonal lineages disappear, resulting in clonal erosion and a reduction in effective population size. Apart from perceivable reductions in clonal and genetic diversity, this typically leads to deviations from the Hardy-Weinberg equilibrium, and increases among-population genetic differentiation as a result of enhanced genetic drift [4][5][6].
During parthenogenetic reproduction in the growing season, genotypes best adapted to their local environment are favoured. This process often results in the coexistence of a limited number of genotypes [7,8]. Clonal diversity is restored by sexual reproduction and the subsequent hatching of recombinant genotypes. Additionally, genetic diversity can increase through hatching of older dormant eggs produced during earlier growing seasons [9]. However, prolonged periods of clonal selection, though benefiting certain clones, may have a negative impact on the population as a whole. Populations where sexual reproduction is infrequent have lower effective population sizes, and sometimes suffer from inbreeding depression [10].
The above-mentioned processes are of course not limited to cladocerans. The genetic structure of cyclical parthenogen populations in general is affected by the strength of the clonal selection, length of the growing season, and frequency of sexual reproduction [1,5]. A reduction of clonal diversity over time has also been observed in natural populations of rotifers (e.g., [11]), aphids (e.g., [12]), as well as plants (e.g., [13]). Similarly, the structure of strictly asexual populations composed of different clones is affected by the frequency of formation or immigration of new clones, and clonal decay [14]. If new clones are not replaced with sufficient frequency, a reduction of clonal diversity over time can be expected due to neutral processes, even in the absence of any selection advantage of particular clones [15].
An important group in which these phenomena have been studied in detail is the cladoceran genus Daphnia. These small crustaceans are key grazers of phytoplankton in temperate lakes. In such habitats, Daphnia have two options to survive unfavourable winter conditions [16]: 1) as dormant eggs, re-colonizing the water column in spring as hatchlings; or 2) as active parthenogenetic females overwintering in the water column. These two strategies are not mutually exclusive, as a Daphnia female may produce dormant eggs sexually and subsequently switch back to parthenogenetic reproduction [17]. Variation in overwintering strategy has been observed both between Daphnia species inhabiting the same lake (e.g., [18]) and within the population of a single species (e.g., [16]). It has been also documented that there is a substantial impact of different recruitment strategies on the genetic structure of a Daphnia population among years with different weather conditions (in particular, warm vs. cold winters) [19]. In the case of a successful overwintering of maternal genotypes to the next growing season, the period of clonal selection may span many years, much longer than for populations relying on regular re-establishment from the dormant egg bank [4,5]. Individual-based modelling [6] predicts that the length of the parthenogenetic reproduction phase in the Daphnia life cycle may be crucial for the resulting clonal diversity. Therefore, different overwintering strategies are expected to have very different impacts on the clonal structure of Daphnia populations, their effective population size, and in the long term on their microevolutionary potential [2].
Accurate analyses of Daphnia clonal diversity, however, have long been hindered by a lack of suitable genetic markers. Most studies in the past have used allozyme electrophoresis with limited genetic resolution to detect multilocus genotypes (e.g., [8,19,20]). While this method has brought valuable insights into processes shaping populations diversity and structure, it is likely that the clonal variation in natural populations was often substantially underestimated [21]. The development of variable microsatellite markers for Daphnia species has allowed much more sensitive genetic analyses, and increased the chances that different clones are properly identified, and true clonal diversity better estimated (e.g., [16,21,22]).
In this study, we focus on the variation in clonal structure of two Daphnia species inhabiting mountain lakes in relation to their presumed overwintering strategy. We studied six Daphnia populations from lakes in the Tatra Mountains (20°10'E, 49°10'N; the highest mountain range of the Carpathians, on the border between Slovakia and Poland; Figure 1) belonging to two closely related species of the Daphnia longispina complex, D. galeata and D. longispina (see [23] for phylogeny and nomenclatural issues in this complex). Both studied species are found in several mountain lakes of the region [24]. These taxa reproduce by cyclical parthenogenesis, and overwintering strategies for several local populations are known. In three lakes inhabited by D. galeata, individuals have been recorded under the ice, while in another locality inhabited by D. longispina no such individuals have been found (Table 1, Figure 1). This latter population presumably survives the winter in the form of dormant eggs only, although overwintering of a small number of females, with densities below the detection threshold, can never be completely excluded.
We assume that similar strategies may occur in other populations living in environmentally similar conditions.
The survival of parthenogenetic females, even at low densities, may have profound consequences for population structure, as such females have a short-term advantage at the beginning of the growing season compared to dormant eggs [5]. This advantage may be further enhanced if numerous individuals of the same clone   overwinter, as can be expected after a prolonged period of clonal erosion. Using nine microsatellite loci, we assessed the late-summer clonal structure in three populations of each of the above-mentioned Daphnia species in order to reveal whether presumed differences in the duration of clonal reproduction (due to different overwintering strategy) are reflected in clonal richness and diversity.

Results
Populations of both species strikingly differed in their clonal composition (Table 1, Figure 2 The dominant MLG in these populations was heterozygous at five out of nine loci, making it highly unlikely that it harbours a substantial number of genotypes produced by sexual reproduction within the clone (i.e., due to selfing). Moreover, the percentage of polymorphic loci and allele richness in those populations was sufficient to detect possible sexually-produced MLGs. This was confirmed by the analysis of MLG richness and diversity of the randomised datasets simulating sexual reproduction within populations with the same MLG structure as recorded in the field (Table 1). Average values obtained from 25 "artificial hybrid" samples (N = 40) based on each of these two D. galeata populations were much higher for both clonal richness (0.88 and 1.00) and diversity (0.97-1.00) than in the original samples, approaching rather those observed in D. longispina populations. Furthermore, only 2.7 to 3.8% out of 1000 simulated "artificial hybrids" had MLGs identical to the respective dominant clones.
The individuals that exhibited different MLGs in these two lakes differed from the dominant ones at one locus in Nižné Žabie Bielovodské and at two loci in Morskie Oko, respectively; one allele at those variable loci was always shared with the dominant MLG. Five and four loci that were heterozygous in the dominant clones were also identical in the rare MLGs. Such differences correspond to distance classes 2 and 8, respectively, between dominant and rare MLGs, as calculated in the software GenoType. These are relatively small values; identical or lower distances between pairs of 1000 randomised MLGs were observed in only 1.8 and 5.3% of pairwise comparisons, respectively.
The third D. galeata population (from Štrbské Lake) was more variable than the other two, with observed values of clonal richness and diversity reaching 0.50 and 0.91, respectively. Several MLGs were detected multiple times in the sample. The two most common ones were detected seven and nine times, reaching respective proportions of 18 and 24% in the analysed sample of 38 individuals. These two locally dominant clones were heterozygous at nine and eight loci, respectively.
All three D. galeata populations showed significant deviations from HWE, with a strong heterozygote excess as a result of the dominance of heterozygous clones. The observed among-population differentiation expressed as the D est value was even higher than in D. longispina (0.42).

Discussion
The populations of two related Daphnia species from the same mountain range strongly differed in their degree of clonal diversity, consistently with assumptions of their overwintering strategy. The differences in population structure between the two groups agree well with predictions of a physiologically-structured model of Daphnia life-history [6], which assessed the impact of clonal erosion on the genetic structure as observed by neutral markers.
Actively overwintering populations of D. galeata, which undergo a prolonged period of clonal selection, had much lower levels of clonal diversity, being almost completely dominated by a single clone in two cases. Moreover, the single individuals in these lakes that exhibited different multilocus genotypes differed only slightly from the dominant clones, being identical at a number of heterozygous loci. It is therefore likely that these individuals did not represent other hatchlings from sexually-produced dormant eggs but the observed differences were rather due to PCR artefacts or possibly somatic mutations of the dominant clones. This is particularly likely for Nižné Žabie Bielovodské, in which the rare MLG differed from the dominant MLG by a single allele of one locus.
In contrast with the situation in D. galeata, intrapopulation variation of D. longispina was very high, similar to what could be expected in an obligately sexual species (as demonstrated by the analysis of datasets simulating sexual reproduction in D. galeata; see also [4]). Although we only have data on the absence of individuals under ice for one of the three studied D. longispina populations, we can assume that all three populations most likely overwinter as dormant eggs, as suggested by similar genetic variation patterns. High Figure 2 Factorial correspondence analysis of all detected Daphnia multilocus genotypes, based on allelic variation at 9 microsatellite loci. MLGs that were detected in more than 5 individuals are shown as enlarged symbols. Note that the two points representing MLGs from Morskie Oko (MO) overlap. Lake abbreviations are explained in Table 1. clonal diversity suggests the limited influence of clonal erosion and the strong impact of sexual reproduction, which is consistent with the yearly re-establishment of the active population from dormant egg banks [5].
The observed patterns of clonal variation suggest that most populations we studied (apart from Štrbské Lake) represent extreme cases in a continuum of overwintering strategies. The contribution of individuals surviving the winter period to the next season's population may substantially differ, both among different localities (as observed by us) and among years within the same locality [19]. The overwintering clones may belong to the most successful ones within the population. The persistence of several genotypes during two years was observed for Daphnia longispina in an artificial German lake [22]; two such MLGs were the most common in both studied years (ranging from 11 to 16% of all individuals in a particular sample). We assume that this represents an intermediate situation in which both overwintering clones and new hatchlings substantially contribute to the genetic structure, a pattern probably similar to the one observed for D. galeata in Štrbské Lake.
A wide range of clonal diversities were observed for multiple populations of the D. longispina complex from various European habitats analysed by Thielsch et al. [21]. The pattern was congruent with our observationsthe majority of D. longispina populations were characterised by very high clonal richness and diversity, but two out of three populations of D. galeata included in the analysis showed reduced variation. One of these was from a Dutch lake, in which we assume Daphnia may indeed persist year-round due to the relatively mild oceanic climate. However, low MLG diversity does not always correspond to long-term or strong clonal erosion. For example, the lowest diversity among D. longispina populations was reported from a small Tatra Mountain lake, Vyšné Satanie [21]. In that case, however, it was most likely due to low allelic richness and low heterozygosity (and thus limited ability to differentiate between different MLGs), as it is unlikely that harsh winter conditions in this lake allow overwintering of cladocerans in active stages. The low allele richness in that particular population more probably results from an introduction bottleneck when the population became re-established after a period of severe anthropogenic acidification [21,24].
Although we studied two different species, the overwintering strategy in the D. longispina complex is not necessarily species-specific. Whether or not Daphnia successfully survive the winter period is rather influenced by the ability to adapt to local conditions in a particular lake [18,25,26]. For example, populations of the same two species co-occurring in Lake Constance showed the opposite pattern to that observed in the Tatra Mountains: D. longispina (labelled D. hyalina in previous studies) overwintered in Lake Constance and did not invest in sexual reproduction, while D. galeata produced dormant eggs and disappeared from the water column in winter [18]. This was also reflected in MLG variation (based on two allozyme loci) being lower in overwintering D. longispina than in D. galeata [18].
The fact that two coexisting Daphnia taxa in Lake Constance differed in their overwintering strategy within a single lake suggests that this life history trait has a genetic component. Although the conspecific Tatra Mountain populations analysed here showed a similar genetic structure, this is not necessarily a consequence of genetic relatedness. The patterns of mitochondrial variation suggest that each of these species colonised the Tatra Mountains multiple times, and in only two of the studied lakes from the West Tatras, Dolné Roháčské and Vyšné Jamnícke, did they likely originate from the same source [24,27]. This pattern is well reflected in the Factorial Correspondence Analysis plot (Figure 2), in which individuals from these lakes are most similar. Furthermore, the overwintering strategy of D. galeata tends to be similar despite the fact that some important selection factors, such as predation pressure, strongly differ among the lakes. In two of them (Morskie Oko and Štrbské Lake), fish predation on Daphnia is high [24,29], while there are no fish in the third studied lake (Nižné Žabie Bielovodské).
The strategy of daphnids in the Tatra Mountain lakes is most likely directly influenced by the size and depth of lakes. In this mountain range, D. galeata inhabits several relatively large and deep lakes, while D. longispina is found in smaller ones [24]. In deeper lakes, the likelihood of successful overwintering of Daphnia individuals is higher, as they may survive in the deep refuge [25]. In addition, investing into dormant eggs is much less efficient, as ephippia that sink to deep parts of the lakes are much less likely to hatch [28]. The studied D. galeata populations from the Tatra Mountains apparently regularly overwinter, as observed in previous studies (Table  1). In fact, investment in sexual reproduction and ephippia production seems to be absent or extremely low in the deep lake Morskie Oko, where no ephippia-bearing females or males were observed during an intensive one-year study of this population [29].
Apparently, prolonged clonal reproduction is highly advantageous for D. galeata populations in the studied lakes. Gliwicz et al. [29] even suggested that Morskie Oko Daphnia are obligately asexual. Indeed, a higher frequency of obligate parthenogenetic Daphnia has been observed at high altitudes [e.g., [2,30]], and populations identified as D. pulicaria inhabiting alpine lakes in the Tatra Mountains are strictly asexual [31]. Interestingly, even asexual lineages coexisting in a single lake may differ in overwintering strategy [29]. However, we consider it unlikely that D. galeata populations in the Tatra Mountains are obligate parthenogens, in the sense that they produce dormant eggs parthenogenetically (see [2] for an overview). To our knowledge, this reproduction mode has never been convincingly shown in the D. longispina complex, and it is unlikely that it would have arisen at least twice independently in the Tatra Mountain lakes that were likely colonized by D. galeata from different sources [24,27]. It seems rather that local D. galeata populations remain in the parthenogenetic life cycle phase only, and have stopped investing in sexual reproduction altogether.
Moreover, we observed reduced levels of clonal diversity in the D. galeata population from Štrbské Lake, a locality in which the Daphnia history in recent decades is relatively well known. Štrbské Lake was colonized by D. galeata in the second half of the 20 th century, and subsequently gradually replaced the resident population of D. longispina [24,32]. From this locality, we have direct evidence for the production of ephippia in D. galeata, as well-preserved dormant eggs of this species extracted from various sediment layers from this lake were analysed genetically [32]. Interestingly, D. longispina also could be found in Štrbské Lake in winter at the beginning of the 20 th century [33], which suggests generally favourable conditions for overwintering at this locality. Historical data also confirm that D. galeata was already present in Morskie Oko a century ago [24,33]. It is possible that the higher clonal diversity observed in D. galeata from Štrbské Lake is due to the much younger age of this population, as a result of which clonal selection has not yet eroded genetic diversity to the same extent as in the two other populations.
Alternatively, this population may have a generally higher tendency to recruit from dormant eggs than the other two studied D. galeata populations, either due to local environmental factors or due to its genetic background. In particular, it is possible that the local population was influenced by introgression from D. longispina during the period of species replacement (see [34]), as also suggested by the intermediate position of some MLGs from Štrbské Lake in the Factorial Correspondence Analysis (Figure 2). However, we presume that environmental conditions are more likely driving the Daphnia overwintering strategy than the taxonomic composition of the populations.
Apart from reductions of clonal diversity, another predicted consequence of prolonged clonal erosion is deviation from the Hardy-Weinberg equilibrium. Simulation data [6] suggest that the typical result of clonal selection is a heterozygote excess even in the absence of any selection advantage for heterozygotes. Indeed, we observed strong heterozygote excess in all three studied D. galeata populations that survive winter as active animals, in contrast to D. longispina populations. The third consequence of clonal erosion may be among-population differentiation [6]. This was high for both studied species, but D. galeata did show higher differentiation (D est = 0.42, in comparison with 0.33 for D. longispina), which would conform to the prediction. In this case, however, factors other than clonal selection are probably more important, in particular persistent founder effects [4,35]. Patterns of mitochondrial DNA variation suggest multiple independent colonisations of Tatra Mountain lakes within each species [24,27], and the high amongpopulation differentiation is likely a direct consequence.
Our data show that the genetic diversity of a Daphnia population may be strongly influenced by the choice of reproductive strategy during unfavourable periods, and can show greatly different patterns among populations living in close proximity to one another, depending on local environmental conditions. What are the benefits and costs of an active overwintering strategy? Females surviving under the ice are ready to start reproduction as soon as conditions improve in spring, which is a great advantage against genotypes hatching from dormant eggs [26]. If seasonal changes in a particular lake are sufficiently predictable over a long period, overwintering genotypes may eventually prevail in the population if no selection force acts in a negative frequencydependent manner (e.g., microparasites; see [36]). Furthermore, immigrating genotypes less adapted to local conditions would be highly unlikely to establish [37], and even if so, they would not succeed in successful interbreeding with the locally adapted clones.
In extreme cases, long-term clonal erosion may result in the overwhelming dominance of a single clone, as observed in two of the three studied D. galeata populations. However, if these clones invest little or nothing into the production of dormant eggs, this may be an evolutionary trap. Ephippia produced earlier in the population are effectively buried in the sediment, and re-establishment from the dormant egg bank may be difficult (but see [38]). A low effective population size may then prevent microevolutionary changes necessary for adaptation to new selective forces, such as the introduction of new predators or parasites.

Conclusions
Our study demonstrates how different life history adaptations to unfavourable periods may impact the population genetic diversity in cyclical parthenogens. Two closely related Daphnia species living in mountain lakes differed in overwintering strategy, which influences the length of the period of clonal erosion and frequency of sexual reproduction. Consequently, strikingly different patterns of genetic diversity were observed in populations of the two species. The use of microsatellite markers, which are variable enough to reveal true clonal structures in cyclical parthenogen populations, revealed that populations surviving unfavourable conditions as active individuals may be dominated by a single clone. Such an extreme reduction of clonal diversity due to long-term clonal erosion may have profound impacts on the microevolutionary potential of such populations.

Methods
There are over 250 glacier lakes in the Tatra Mountains, but only a minority of these are inhabited by Daphnia populations. The two studied species are locally the commonest members of the D. longispina complex (more details on zooplankton of the Tatra Mountain lakes are given in [24,39]). We studied six lakes, three inhabited by D. longispina, and three by D. galeata (Figure 1).
During winter zooplankton sampling of Vyšné Furkotské Lake, inhabited by D. longispina, no individuals were found [40]; likely, this population survives winter as dormant eggs encapsulated in protective ephippia in the lake sediment. Winter zooplankton was never studied in the other two D. longispina lakes, Vyšné Jamnické and Dolné Roháčské (E. Stuchlík, pers. comm.), but given their similarity to Vyšné Furkotské Lake (Table 1), it is likely that their Daphnia populations are under similar selection pressures regarding overwintering strategy. In contrast, all three D. galeata populations, which were sampled from larger and deeper lakes with slightly lower elevation than D. longispina, have been documented to overwinter as adult females under the ice cover (see references in Table 1); in at least one case, they were able to reproduce in such conditions [29].
All studied lakes were sampled in September 2007, close to the end of the main growing season (which is delayed and much shorter in these alpine environments in comparison with lowland habitats). Sampling during this period ensured that clonal structure that might have arisen by hatching early in the growing season (which lasts, depending on altitude, between approx. May/June to October) would be noticed genetically. Samples were collected by tows of a plankton net from the lake shore or from a rubber boat by vertical hauls from the depth, and preserved in 96% ethanol. DNA from individuals was extracted in 100 μl proteinase K solution, using the protocol from [41].
Genotypes of individuals were determined by analysis of nine microsatellite loci described previously [42], amplified in a single multiplex reaction. PCRs of the 11 μl volume consisted of 10 μl of master-mix (2.9 μl of primer mix: 0.4 μM Swid15, 0,3 μM Swid1, 0.05 μM Dp281NB, 0.2 μM Dp196NB, 0.3 μM Swid12, 0.3 μM Dp512, 0.2 μM Swid10, 0.3 μM Swid14, 0.3 μM Dgm109; 5.5 μl of MP-mix (Quiagen Multiplex PCR Kit); 1.6 μl of water) and 1 μl of DNA extract. The PCR cycle consisted of the following steps: initial denaturation at 95°C for 15 min, 30 cycles of denaturation at 94°C for 0.5 min, annealing at 54°C for 1.5 min and elongation at 72°C for 1 min, with final elongation at 60°C for 30 min. Fragment analysis of PCR products was performed on the capillary sequencer ABI 3130 with the Gene Scan Liz 500 size standard, and individuals were subsequently genotyped in GeneMapper 4.0. (Applied Biosystems, Foster City, CA). Individuals differing from dominant MLGs in D. galeata populations were carefully checked to rule out scoring errors.
Each individual was characterized by its multilocus genotype (MLG), and the patterns of MLG similarity were visualised by factorial correspondence analysis (FCA) calculated in Genetix v4.03 [43]. Clonal richness was expressed as the number of MLGs detected in the sample divided by the total number of studied individuals in each population (MLG/N), the clonal diversity as a complement of the maximum likelihood estimator of Simpson's index (1-D), calculated in the program SPADE [44].
To compare the observed clonal structures in each D. galeata population with those expected under the strong influence of recruitment from dormant eggs, we used the program HYBRIDLAB [45] to generate a randomised dataset simulating the result of sexual reproduction of analysed individuals, i.e., multilocus genotypes of artificial hybrids (assuming independent segregation of studied microsatellite loci). We then calculated the above-mentioned measures of clonal richness and diversity for 25 sets of 40 "artificial hybrids" from each D. galeata population, and compared the averaged values with those from field samples of both Daphnia species.
Additionally, we evaluated whether rare genotypes observed in populations dominated by a single MLG more likely arose independently, or resulted from a somatic mutation or methodological artefact. The software GenoType [46] was used to calculate a pairwise distance matrix between individual multilocus genotypes within each sample, assuming a stepwise mutation model. The distribution of such pairwise distances may be used to select a threshold that defines the maximum difference between two MLGs at which they are still assigned to the same clonal lineage [47], i.e., to neglect differences caused by PCR artefacts, scoring errors, or somatic mutations. We compared the distance class distribution of simulated datasets of 1000 "artificial hybrids" (generated as above) with that observed in D. galeata populations dominated by a single clone.
The extent of deviations from the Hardy-Weinberg equilibrium, which are predicted under the scenario of strong clonal erosion [6], were tested in the software Hwclon [7] using Monte Carlo simulations (20 batches of 500 permutations). As there were six repeated tests, we applied sequential Bonferroni correction when assessing the significance of the results. Genetic differentiation among populations, based on the evaluated microsatellite markers, was assessed by means of D est [48], using the SMOGD software [49].