Region | Forward primer | Reverse primer | Annealing temperature |
---|---|---|---|
ex5-in6 (PCR 1) | 5F2898: TTGACAGCCTYGATGATGAT | Svex7R3420: ATCTTGAAAATCCAGCCC | 52°C |
ex5-in6 | 5F3062: AATGATGASGGGAAGAATTTTGC | Svex7R3420 | 52°C |
ex6-ex7 (PCR 2) | vex6F3263: GYCARCTYCTTGAGGCTGC | 7R3883: ATVCCCATGCTGAAKAGCTCYTG | 59°C |
ex5-ex6 | 5F2898 | vex6R3371: YMTCRACACTGGGAGTGGAG | 54°C |
ex5-ex6 | 5F3062 | vex6R3371 | 57°C |
ex6-in6 | vex6F3263 | Svex7R3420 | 52°C |
ex7 | vex7F3418: GGCTGGATTTTCAAGATGG | 7R3883 | 55°C |