Conservation level | dN/dS | Adjusted dN/dS | Â | Synonymous | Non-synonymous | Chi-squared | Neutrality Index |
---|
Identical | 0.030 | 0.034 | D | 839 | 101 | 8.3 × 10-15 | 5.6 |
 |  |  | P | 52 | 35 |  |  |
Semi-conserved | 0.21 | 0.24 | D | 186 | 174 | 0.22 | 1.6 |
 |  |  | P | 12 | 18 |  |  |
Non-conserved | 0.35 | 0.39 | D | 90 | 118 | 0.38 | 1.5 |
 |  |  | P | 7 | 14 |  |  |
Non-aligned | 0.55 | 0.64 | D | 155 | 402 | 0.74 | 1.1 |
 |  |  | P | 12 | 35 |  |  |
Total | 0.15 | 0.17 | D | 1270 | 795 | 9.5 × 10-6 | 2.0 |
 |  |  | P | 83 | 102 |  |  |
- dN/dS ratios refer to P. falciparum /P. reichenowi divergence. Conservation levels were based on BLOSUM62 score in P. falciparum - P. chabaudi alignments; identical (+4 and above), semi-conserved (0 to +3), non-conserved (-4 to -1) and non-aligned. Adjusted dN/dS ratios were derived based on the transition: transversion ratio measured at 4-fold degenerate sites (see Table 4). D = divergent mutations, P = polymorphisms. Chi-squared and Neutrality Index are the outputs of the McDonald-Kreitman test; neutrality index indicates the extent to which the levels of variation depart from the expected in the neutral model with values greater than 1 indicating an excess of polymorphism and hence negative selection.