Figure 1

A-D – Phylogenetic trees for four of the investigated gene families. Phylogenetic neighbor-joining trees for four of the gene families analyzed in this study (A: IGFBP, B: NFE, C: AOC and D: G6PC). All trees constructed with the NJ method as implemented in Clustal W 1.81 with 1000 bootstrap replicates (values shown for each node). For IGFBP, sequences without the domains PF00219 (Insulin-like growth factor binding protein) and PF00086 (Thyroglobulin type-1 repeat) were removed from the alignment. Note that the tree contains two subfamilies relevant for this paralogon. Hsa is human (Homo sapiens), Mmu: Mouse (Mus musculus), Dre: zebrafish (Danio rerio), Tru: fugu (Takifugu rubripes), Tni: tetraodon (Tetraodon nigroviridis), Cin: (Ciona intestinalis), Brf (Branchiostoma floridae) and Dme: (Drosophila melanogaster). For complete phylogenetic analysis of all 14 families see Additional files 1 and 2.