Genetic diversity of Ophiocordyceps sinensis, a medicinal fungus endemic to the Tibetan Plateau: Implications for its evolution and conservation

Table 1 Comparison of genetic diversity parameters^a

Populations^b	No. of sequences	No. of polymorphic sites^c	Average no. of nucleotide differences, K	Nucleotide diversity, Pi(×10^-2)	Haplotype diversity, h
Qilian (N)	2	0 (0)	0.00 (0.00)	0.00 (0.00)	0.00 (0.00)
Laji (N)	2	1 (0)	1.00 (0.00)	0.19 ± 0.09 (0.00)	1.00 ± 0.50 (0.00)
Yushu (N)	6	1 (1)	0.33 (0.33)	0.06 ± 0.04 (0.04 ± 0.02)	0.33 ± 0.22 (0.33 ± 0.22)
Nagqu (N)	12	2 (1)	0.58 (0.17)	0.11 ± 0.03 (0.02 ± 0.01)	0.53 ± 0.14 (0.16 ± 0.13)
Chamdo (N)	9	1 (0)	0.22 (0.00)	0.04 ± 0.03 (0.00)	0.22 ± 0.17 (0.00)
Nyingchi (S)	11	22 (23)	6.51 (7.42)	1.25 ± 0.44 (0.85 ± 0.27)	0.62 ± 0.16 (0.80 ± 0.11)
Mila (S)	2	7 (4)	7.00 (4.00)	1.31 ± 0.66 (0.46 ± 0.23)	1.00 ± 0.50 (1.00 ± 0.50)
Garze (S)	5	2 (5)	0.80 (2.00)	0.15 ± 0.09 (0.23 ± 0.11)	0.40 ± 0.24 (0.70 ± 0.22)
Baima (S)	5	1 (2)	0.60 (1.00)	0.11 ± 0.03 (0.11 ± 0.03)	0.60 ± 0.18 (0.80 ± 0.16)
Northern regions	31	10 (2)	1.06 (0.13)	0.20 ± 0.07(0.02 ± 0.01)	0.49 ± 0.11 (0.13 ± 0.08)
Southern regions	25	26 (27)	4.37 (5.01)	0.82 ± 0.26 (0.57 ± 0.17)	0.83 ± 0.06 (0.87 ± 0.04)
Overall	56	35 (28)	4.45 (3.35)	0.84 ± 0.13 (0.38 ± 0.09)	0.78 ± 0.05 (0.67 ± 0.06)

^a Fifty-six individuals were sequenced in this study (Additional file 1). "N" or "S" inside parentheses represents northern or southern populations, respectively (the same hereinafter). Numbers outside parentheses are values of ITS, and numbers inside parentheses are values of MAT1-2-1 (the same hereinafter). ^b Genetic parameters of the Shannan population and the unknown population (Additional file 1) are included in the "southern regions" and "overall" statistics, but they are not given individually because they each possess one isolate.^c The "polymorphic sites" include both informative sites and singleton sites. For both ITS and MAT1-2-1, there is one polymorphic site shared by northern and southern isolates.

ISSN: 2730-7182