Evolution and expression of the phosphodiesterase 6 genes unveils vertebrate novelty to control photosensitivity

Table 3 List of the primer pairs used for qRT-PCR experiments and to synthesise riboprobes

qRT-PCR primers
Gene	Forward primer 5′–3′	Reverse Primer 5′–3′	Product length
pde6a	CAGTCAACAAGATCGGGGCT	GCTCAGGTGAAACACTCGGA	104 bp
pde6b	ACTCACGACAGGCAAACTGA	CATGCAGCTTGGCTAGAGGA	146 bp
pde6c	ACTCCTGATGGCAGGGAGAT	AGCAACATAGGTGGGCAGTC	135 bp
pde6ga	CACAAGGGCCCACCTAAGTT	AACTCCAGGTGACTGTACGC	164 bp
pde6gb	GTTCAAGAGCAAGCCCCCAA	GTGCCTAAACCTTCCATGCC	75 bp
pde6ha	CTTCGGAGACGACATCCCAG	ATCGCTGAGCTCCATGTCTC	94 bp
pde6hb	CCTGGACAGAAAGGGTTTGGT	CTGAGCTCCATGTCCCCGAA	104 bp
pde6i	ACAACTACACCCAGAAGCGG	TGCCAAGACCATCCATTCCT	127 bp
actb1	GGCACGAGAGATCTTCACTCCCC	CCATGCCAACCATCACTCCCTGA	195 bp
tuba1b	CGGAGCTGGAAAACACGTCCCC	TGGTCAGACAGTTTGCGAACCCTA	216 bp
Probe primers
pde6ga	TCCACCAGCAACATCCTGCACC	CGCGCTATGGCAGACGCTGA	679 bp
pde6gb	TCTGCCATGTCCTCCATCGGC	AGACGAGGCACCGAGGCACA	381 bp
pde6ha	GGCGCTCTCAGGCCAACACA	AGGCACAAACACAATCTCATGCACA	205 bp
pde6hb	TGGCCAAATACGGCATCATCT	CATCCATCGTGGCTGCTACA	280 bp

The current table specifies the primer pair sequences used to amplify gene sequences either to perform qRT-PCR or to synthesise the antisense and sense riboprobes used in ISH experiments. Actin beta I (actb1) and tubulin alpha 1b (tuba1b) were used as housekeeping genes in the qRT-PCR experiments

ISSN: 2730-7182