Fig. 3

Conservation of the Prp38-MFAP1 interface. a, b Cartoon representation of (a) the heterodimer of hsPrp38 (red) and hsMFAP1 (blue) (PDB ID: 5F5S, [34]) or of (b) the heterodimer of hsPrp38 (red) and hsMFAP1 (blue) (PDB ID: 5F5T, [34]). Key interaction residues are presented as sticks (right panels). Dashed, black lines indicate hydrogen bonds and salt bridges. c Excerpt from a multiple protein sequence alignment of MFAP1 orthologs identified in this study. Key Prp38-interacting residues (R282, R286, R289 according to the hsMFAP1 sequence) are marked; percentage identity is given in brackets. In general, residue color intensity indicates level of sequence identity at that specific position; coloring starts at a sequence identity of 30%. Blue - conserved hydrophobic residues; red – conserved positively charged residues; purple – conserved negatively charged residues; green – conserved glutamines; cyan – conserved histidines. H. sapiens MFAP1, K. lactis Spp381 and S. cerevisiae Spp381 are highlighted by boxes