Table 3 Summary statistics for the 18 nSSR loci used to genotype Scots pine. Abbreviated values from left to right include the total detected alleles (N), allelic richness (A), observed heterozygosity (Ho), expected heterozygosity (He), inbreeding coefficient (FIS) and genetic diversity which apportions between populations (FST). FIS and FST values are calculated according to Nei (1987) and Weir & Cockerham (1984). The latter are unbiased by sample size differences. FIS and FST values in bold are significantly greater than zero. (*p ≤ 0.05; **p ≤ 0.01; p*** ≤ 0.001). Loci with null frequency values in bold were removed from analyses where indicated
Locus | N | Allele size range | A | H o | H e | FIS(Nei) | FIS(WC) | FST(Nei) | F ST(WC) | Null frequency |
|---|---|---|---|---|---|---|---|---|---|---|
PtTX3116 | 26 | 116–278 | 1.803 | 0.458 | 0.869 | 0.453*** | 0.459*** | 0.038** | 0.030*** | 0.175 |
PtTX3032 | 49 | 275–512 | 1.916 | 0.874 | 0.954 | 0.088*** | 0.076*** | 0.012 | 0.010* | 0.026 |
psy119 | 5 | 310–322 | 1.130 | 0.100 | 0.104 | 0.013 | 0.019 | 0.035 | 0.027 | 0.005 |
SPAC11.6 | 45 | 108–220 | 1.881 | 0.615 | 0.926 | 0.346*** | 0.328*** | 0.016 | 0.016** | 0.135 |
psy117 | 8 | 215–229 | 1.642 | 0.230 | 0.691 | 0.672*** | 0.659*** | 0.030* | 0.030** | 0.251 |
SPAC12.5 | 30 | 122–190 | 1.879 | 0.901 | 0.914 | -0.016 | -0.010 | 0.020* | 0.021*** | 0.019 |
SPAG7.14 | 55 | 174–244 | 1.900 | 0.808 | 0.944 | 0.117*** | 0.126*** | 0.025*** | 0.024*** | 0.037 |
PtTX3107 | 12 | 144–171 | 1.723 | 0.379 | 0.765 | 0.465*** | 0.503*** | 0.009 | 0.004 | 0.182 |
PtTX4001 | 15 | 196–226 | 1.755 | 0.762 | 0.781 | 0.014 | 0.005 | 0.027* | 0.023** | 0.021 |
PtTX4011 | 9 | 231–277 | 1.530 | 0.441 | 0.583 | 0.227*** | 0.207*** | 0.062*** | 0.050*** | 0.075 |
psy118 | 7 | 287–317 | 1.134 | 0.111 | 0.120 | 0.058 | 0.068 | 0.009 | 0.009 | 0.010 |
psy12 | 4 | 197–206 | 1.303 | 0.255 | 0.302 | 0.140* | 0.141* | 0.024 | 0.019 | 0.769 |
psy125 | 2 | 212–215 | 1.042 | 0.038 | 0.043 | 0.087 | 0.086 | 0.019 | 0.034 | 0.312 |
psy136 | 5 | 244–256 | 1.098 | 0.122 | 0.117 | -0.038 | -0.045 | 0.000 | 0.000 | 0.000 |
psy142 | 6 | 165–175 | 1.648 | 0.659 | 0.677 | -0.002 | 0.011 | 0.020 | 0.018 | 0.030 |
psy144 | 5 | 164–175 | 1.148 | 0.120 | 0.144 | 0.043 | 0.078 | 0.103* | 0.105** | 0.014 |
psy157 | 6 | 184–199 | 1.388 | 0.369 | 0.414 | 0.036 | 0.050 | 0.086*** | 0.066*** | 0.021 |
SPAC11.4 | 20 | 132–170 | 1.811 | 0.842 | 0.852 | -0.015 | -0.015 | 0.035*** | 0.025*** | 0.016 |
Mean | 17 | - | 1.541 | 0.449 | 0.567 | 0.149*** | 0.156*** | 0.032*** | 0.028*** | 0.117 |