Table 3 Sequences of primer pairs with Nextera Illumina adaptors. Target, product size, and reaction conditions for real-time PCR assays were the same for both primers

Forward Primer V418SNextFor:

18 S rRNA

V4

378 bp

5 min at 95 °C,

30 × (30 s at 95 °C, 30 s at 55.2 °C, 30 s at 72 °C),

5 min at 72 °C.

 5’-[TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG]

 CCAGCASCYGCGGTAATTCC-3’

Reverse primer V418SNextRev:

 5’-[GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG]

 ACTTTCGTTCTTGATYRATGA-3’