Table 1 Insect beta tubulin sequence features.
Isoform | Function | Average and maximum pairwise distances | -COOH terminus sequence | Post-translational modification sites/Conserved sequence features | ||
|---|---|---|---|---|---|---|
| Â | Â | Drosophila | Mosquito | All Insects | Â | Â |
β1 | Major isoform | 0.000 +/- 0.000 (n = 12) | 0.002 +/- 0.002 (n = 2) | Average 0.011 +/- 0.003 (n = 9) Maximum 0.027 (n = 10) | Ha β1a EATADDEAE FEEEGEVEGE YA Ha β1b EATADDEAE FEEEGEVEGE YD Dm β1 EATADEDAE FEEEQEAEVD EN Ae β1 EATADEDAE FDEEQEAEVD EN Ag β1 EATADEDAE FDEEQEAEVD EN Bm β1a EATADEDAE FDEEQEQEIE DN Bm β1b EATADEDAE FDEEQEQEIE EH Tc β1 EATADEDAE FDEEQEAEVD EN Nv β1a - Nv β1b TMNGPRDAP DEDVEVVEEE LRD Am β1 EATADEDAE FDEEQEAEVD EN Ap β1 EATADEEAE FDEEQEQEVD EN Ph β1 EATADEDAE FDEEQEEVVD EN | PTM sites Polyglutamylation - yes Polyglycylation - yes Phosphorylation - yes |
β2 | Testis-specific isoform | 0.000 +/- 0.000 (n = 12) | 0.060 +/- 0.011 (n = 2) | Average 0.085 +/- 0.009 (n = 9) Maximum 0.464 (n = 17) | Dm β2 EATADEEGE FDEDEEGGGD E Ae β2 EATADEEGE FDEEEEGGEE Ag β2 EATADDEGE MDEEEEGGED Bm β2 DATADDEGE FDEEAEEGLE E Tcβ2a DATAEEEGE FDEEEEGDNE GEN Tc β2b DAEVDEEYG DEDETEEDKF EEET Nv β2a EATAEEDTE FDEDEGENEG N Nv β2b EATADEFAD YEEDEEEEED YA Nv β2c EATTEE--D FETEDAGDD FETCDQE Am β2a EATAEEEGE FDEEEEGEGE HP Am β2b EATAEDEGE FDEEEETEK Apβ2a DATVDEDGE GDDDEEDADA Apβ2b EATIDETGE-EDEDEDADA Apβ2c DATVDEEGE GDDDDEDAEA Apβ2d EATVDAPGG VNEE Ph β2a EATADEEGE DEEDEGGED Ph β2b EATSYEYDE DEGEENEVEE EEEKEMTNWL PA | PTM sites Polyglutamylation - yes, except Apβ2d Polyglycylation -- yes, except Apβ2d Phosphorylation -- yes Conserved sequence features Axoneme motif - yes, except Agβ2, Tcβ2b, Nvβ2a-c, Apβ2a-d, Phβ2a, b Gly56-- yes, except Phβ2b |
β3 | Minor isoform expressed in variety of pre-adult mesodermal and neural domains | 0.009 +/- 0.002 (n = 10) | 0.014 +/- 0.005 (n = 2) | Average 0.092 +/- 0.010 (n = 9) Maximum 0.148 (n = 9) | Dm β3 EATADDEFD PEVNQEEVEG DCI Ae β3 EATADDEFE QEECADEMEG ECV Ag β3 EATADDEFE QEDCQDEMEG ECV Bm β3 EATAEDDTE FDQEDLEELA QDEHHD Tc β3 EATADEEYE AEEEAAADDF NC Nv β3 EATTEEDFE TEDAGDDFET CDQE Am β3 EATAEEDFE AEECADDFET CDQE Ap β3 EASVDEEYI EEEETEETDM CD Ph β3 LYISTIIKI | PTM sites Polyglutamylation -- yes Polyglycylation -- yes, except Tcβ3, Nvβ3, Amβ3, Nvβ3, Apβ3 Phosphorylation - yes Conserved sequence features Nucleotide-binding domain amino acid insert (aa56) - yes |
β4 | Minor isoform, pre-adult tissues in Dm , absent in Bm) | 0.032 +/- 0.005 (n = 11) | 0.104 +/- 0.012 (n = 3) | Average 0.136 +/- 0.015 (n = 5) Maximum 0.186 (n = 6) | Dm β4 EATADDEVE FDDEQAEQEG YESEVLQNGN GE Ae β4a EASADDYVE GEHDFDDEEE IQQ Ae β4b DASVEDYED GEEMIEEEGE QHVE Ag β4 DAEVEDYDE MEEIPEEEQQ QQQE Ap β4 EATAEEVEF DDEEVVEEVD DNKDY Ph β4 VRSSLHLSN AANIEIQKNE ILNRNT | PTM sites Polyglutamylation - ? Polyglycylation - ? Phosphorylation - yes |