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Table 2 Primers and PCR cycling conditions used in this study.

From: Revisiting the taxonomy of the Rattini tribe: a phylogeny-based delimitation of species boundaries

Designation

Gene Name

Nucleotide sequence 5' → 3'

Annealing Temperature

Fragment Length (bp)

Original Publication

Cytb

cytochrome b

    

L14723

 

ACCAATGACATGAAAAATCATCGTT

50°C

1213

[69]

H15915

 

TCTCCATTTCTGGTTTACAAGAC

   

COI

Cytochrome c oxydase I

    

BatL5310

 

CCTACTCRGCCATTTTACCTATG

48°C

750

[25]

R6036R

 

ACTTCTGGGTGTCCAAAGAATCA

   

IRBP1

Interphotoreceptor retinoid binding protein (fragment 1)

   

I1-Rattus

 

ATTGAGCAGGCTATGAAGAG

58°C

785

this study

J2-Rattus

 

TAGGGCTTGCTCYGCAGG

   

IRBP2

Interphotoreceptor retinoid binding protein (fragment 2)

   

I2

 

ATCCCCTATGTCATCTCCTACYTG

52°C

892

[70]

J1

 

CGCAGGTCCATGATGAGGTGCTCCGTGTCCTG

   

cytb barcode

Cytochrome b (museum specimens)

   

MPLeopol-fw MPRattusSL-Rev

 

GAYAAAATYCCATTCCACCC TARTTRTCYGGGTCTCC

48°C

122

this study

  1. The IRBP gene was amplified into two overlapping fragments, IRBP1 and IRBP2.
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BMC Ecology and Evolution

ISSN: 2730-7182

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