Table 2 Estimation of gene copy number of antimicrobial effectors and non immune gene by real time PCR
From: Molecular diversity of antimicrobial effectors in the oyster Crassostrea gigas
gene | Sample | Primer efficiency (E) | Calculated copy number | Estimated copy number | ||
|---|---|---|---|---|---|---|
| Â | Â | Mean1 | SD | Mean2 | SD | Â |
Cg-bpi | pDNA | 2.01 | ± 0.006 |  |  |  |
|  | gDNA | 2.06 | ± 0.060 | 0.95 | ± 0.10 | 1 |
Cg-prp | pDNA | 1.99 | ± 0.008 |  |  |  |
|  | gDNA | 2.03 | ± 0.040 | 12.59 | ± 0.99 | 11 - 13 |
Cg-defs | pDNA | 2.02 | ± 0.004 |  |  |  |
|  | gDNA | 2.03 | ± 0.010 | 47.46 | ± 6.4 3 | 41 - 53 |
Cg-actin | pDNA | 1.99 | ± 0.030 |  |  |  |
|  | gDNA | 2.07 | ± 0.040 | 168.04 | ± 10. 4 | 156 - 176 |