Expansion of ferlin family occurred during vertebrate evolution. Maximum likelihood tree constructed using protein sequences from ferlin C2F domains. Bootstrap values from 100 iterations are shown for major branches. Lamprey and elephant shark sequences are coloured in blue and orange, respectively. The inclusion of basal vertebrates lamprey (Pmar) and elephant shark (Cmil) demonstrate the expansion of the ferlin family during vertebrate evolution occurred after the divergence of jawless fish (lamprey) but before cartiligous fish (shark).