Chaperonin genes on the rise: new divergent classes and intense duplication in human and other vertebrate genomes

Table 3 Divergence of BBS and CCT8L proteins relative to CCT8 proteins

	MKKS	BBS10	BBS12	CCT8L¹
No. species²	14	11	11	5
Size (W_B)³	5.7770	4.6020	5.8949	3.3859
B-index (B_B)⁴	0.6976	1.1079	1.0284	0.3196
Unbiased pair-wise distance (B_B× 2)	1.3952	2.2159	2.0568	0.6393
L_B(B_B× W_B)⁵	4.0300	5.0987	6.0623	1.0822
Average D_ij(D_B)⁶	2.0951	2.9660	3.2858	0.8017
CCT8 ⁷
Size (W_C)	5.5202	4.5503	4.3647	3.1100
B-index (B_C)	0.1146	0.1373	0.0992	0.0227
Unbiased pair-wise distance (B_C× 2)	0.2291	0.2747	0.1983	0.0454
L_C(B_C× W_C)	0.6324	0.6250	0.4328	0.0706
Average D_ij(D_C)	0.3394	0.3687	0.2709	0.0545
B_B/B_C	6.0873	8.0692	10.3669	14.0793
L_B/L_C	6.3725	8.1579	14.0072	15.3286
D_B/D_C	6.1730	8.0445	12.1292	14.7101
W_B/W_C	1.0465	1.0114	1.3506	1.0887

¹Only the human CCT8L2 branch was included in the tree. The CCT8L1 branch had equivalent length; ²Chaperonin-BBS sequences used in the trees were from the following species (see the legend for Figure 2 for a complete list of abbreviations and species names). MKKS: Bt, Cf, Dr, Ec, Ga, Gg, Hs, Md, Mm, Mmu, Ol, Rn, Tr, Xt; BBS10: Bt, Cf, Dr, Ec, Ga, Hs, Md, Mm, Ol, Rn, Tr; BBS12: Bt, Cf, Dr, Ec, Ga, Gg, Hs, Mm, Ol, Rn, Tr, Xl; CCT8L: Bt, Cf, Hs, Mm, Rn. ³Size is the average number of sequences contained in a cluster over evolutionary time (see Methods); ⁴The B-index measures the average substitutions per site (evolutionary distance) of the sequences within a cluster from their common ancestor; ⁵L is the length of the tree (sum of the lengths of all branches); ⁶Average D_ijis the average pair-wise evolutionary distance of the sequences; ⁷Estimates for CCT8 were computed over corresponding species represented by the sets of MKKS, BBS10, BBS12 or CCT8L proteins (see footnote 2, above).

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