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Table 3 Primers for the selective amplification step of AFLP

From: Genetic population structure of sympatric and allopatric populations of Baltic ciscoes (Coregonus albulacomplex, Teleostei, Coregonidae)

Primer combination MseI Primer EcoRI A EcoRI B Number of loci per primer pair
1 CAT ACTfam AAGjoe 208
2 CAG ACTfam AAGjoe 209
3 CTA ACTfam ACAjoe 214
  1. All primers had 3 additional bases on the 3' end. EcoRI primers A and B were labelled with different fluorescent dyes (fam and joe) and 3 different multiplex reactions were run with two forward and one backward primer each.