Figure 3
2ndpart of a series of horizontal vibratome sections of the brain of Speleonectes tulumensis . (A-D) Dorsal to ventral series of vibratome sections (50 μm) through the brain of Speleonectes tulumensis triple labeled for serotonin immunoreactivity (5HT-IR, green), acetylated α-tubulin immunoreactivity (TUB-IR, magenta) and nuclear marker (NUC, blue). Maximum projections consisting of 31 to 41 confocal laser-scans per slice. Abbreviations: capital letters A, D: soma clusters; DCC: deutocerebral commissural neurites; LAN2: lateral antenna 1 neuropil 2; MAN: medial antenna 1 neuropil; ON: olfactory neuropil; sA2, sD1, sD2, sE1, sE2: 5HT-ir cell groups; TCN: tritocerebral neuropil.