Figure 3

A: Evidence for reintegration of a segment similar to CvBV S27 and CcBV Segment 10 in C. sesamiae Mombasa (CsM) and Kitale (CsK) strains. CvBV S27: Expected proviral form of CvBV segment 27 flanked by Direct Repeat Junctions DRJ (black arrowheads). DRJ are involved in the production of the packaged circles from the proviral form. CsM 4B19: Genomic map of viral sequences inserted within a Maverick mobile element in CsM. Only one DRJ is present in the viral sequence indicating that the sequence does not correspond to the proviral form but probably to a virus circle reintegrated into the wasp genome (P3, P2, PRO, Cl, PolB, ATPase, Cc, INT, POLB: conserved genes of insect Maverick elements, Dupuy et al. (2011) [27]). CsK 9 L12: Genomic map of another segment highly similar to CvBV S27 and CcBV S10 reintegrated in a different genomic location, rich in gypsy-related retrotransposable elements in CsK. B: Evidence for reintegration of a segment homologous to CvBV S2 and CcBV S26 in CsM. Symbols are the same as in A. A and B: Alignments of the extremities of the reintegrated segments with homologous sequences of CcBV S10 and CvBV S27 (A) and CcBV S26 and CvBV S2 (B). The re-integrated segments have been cut at the same position during the integration process suggesting that circle reintegration involved a specific mechanism (see Figure 4). The red arrows indicate the position of the reintegration site relative to the DRJ on the circle. Junction sequences are indicated in bold.