Quantification of teleost-specific Atlantic cod tlr s in response to bath challenge with V. anguillarum. Heatmap representing the expression of Atlantic cod tlrs in head-kidney, gills and spleen in response to bath challenge with V. anguillarum. After collecting initial control samples, fish were subjected to bath challenge with V. anguillarum strain H610 at a concentration of 2.6·107 cfu·ml-1. Samples were collected at 4 (4 hpc) and 48 (48 hpc) h post-challenge. Relative expression of tlr21, tlr22 and tlr23 was determined by qPCR and expressed as ratios between each sample and the respective initial control. Significance levels were set at P < 0.05 and statistically different expression values are enclosed in red boxes. Eef1a and ubi were used as internal controls.