Figure 5

Multiple perforin genes were present at distinct loci in the LCA of teleost fish. The Bayesian inference phylogenetic tree of fish perforin homologs was made using the alignment in Additional file 4: Figure S3. Posterior probabilities for major clades are shown in italics. Sequences with evidence of expression are denoted by the letter ‘e’ to the right of relevant tip labels. The clades formed reflected the loci to which genes belong, as shown by a representative scaffold to the right of each clade. Related branches and scaffolds are color coded. Proteins are labelled as the three letter genus/species abbreviation followed by the relevant perforin gene number. Genus/species abbreviation are: Homo sapiens, Hsa; Takifugu rubripes, Tru; Tetraodon nigroviridis, Tni; Danio rerio, Dre; Carassius auratus langsdorfii, Cau; Oryzias latipes, Ola; Gasterosteus aculeatus, Gac; Salmo salar, Ssa; Ctenopharyngodon idella, Cid; Oncorhynchus mykiss, Omy; Paralichthys olivaceus, Pol.