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Table 4 Primer combinations of selective amplification

From: Effects of predation pressure and resource use on morphological divergence in omnivorous prey fish

Primer combination

MseI-Primer

EcoRI-Primer

Number of loci

1

MseI + CAG

EcoRI + ACT + 6FAM™

133

2

MseI + CAG

EcoRI + ACA + VIC®

139

3

MseI + CAG

EcoRI + AAG + NED™

107

4

MseI + CTA

EcoRI + AAG + PET®

68

  1. All primers had 3 additional base pairs at the 3’end. EcoRI-Primers were also labeled by a fluorescent dye at the 5’end.
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BMC Ecology and Evolution

ISSN: 2730-7182

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