Figure 1

Sampling localities, phylogenetic relationships, and distribution of mitochondrial haplotypes and haploclades. Maximum Likelihood (ML) trees depicting the phylogenetic relationships among 75 combined mitochondrial sequences (cytb and nd4) of Podarcis muralis from 52 localities (A) and among 185 cytb sequences of Podarcis muralis obtained in this study (N=82) and from GenBank, (N=103) (see Additional file 1) (B). ML bootstrap values over 1000 replicates are reported in correspondence to the nodes; black stars indicate BA posterior probabilities = 1.00; Podarcis liolepis was used as an outgroup. In the combined cytb/nd4 ML tree specimen codes are reported (see Table 1 for details) and main mitochondrial clades numbered from 1 to 17; in the cytb ML tree tip nodes are collapsed and main mitochondrial clades are numbered from 1 to 23 according to the combined cytb/nd4 ML tree and named according to previous studies. Samples used in this study and geographic distribution of mitochondrial clades are reported in the map: big circles show the 53 localities sampled in this study and triangles show the geographic origin for the cytb sequences obtained from GenBank, coloured according to mitochondrial clades defined by the ML trees (black triangles indicate admixed populations for which the frequency of haplotypes belonging to each clades is shown by the pie diagrams; grey circles indicate samples for which only nuclear sequences were available).