Figure 4
Identification of fatty acid elongation products in transgenic yeast ( S. cerevisiae ) transformed with pike Elovl5 ORF and grown in the presence of LC-PUFA substrates. Yeast were incubated in presence of PUFA substrates Ī±-linolenic acid (ALA, 18:3n-3), stearidonic acid (SDA, 18:4n-3), or eicosapentaenoic acid (EPA, 20:5n-3), and fatty acid elongation products determined. Each panel shows the main endogenous fatty acids of S. cerevisiae, namely 16:0, 18:0, 16:1n-7 and 18:1n-9, and their elongated products 18:1n-7 and 20:1n-9. * Substrates and their corresponding elongated products are indicated accordingly in the panels. Vertical axis, FID response; horizontal axis, retention time.