Dayhoff PAM250 plot for ClustalX-aligned mouse N1 domain amino acid sequence comparisons. At each position in the alignment, the Dayhoff PAM250 log score was determined for pairwise comparisons of each sequence in the set against all the others in the set. Mean and standard deviation were calculated for scores at each residue position. Regions representing the CFG face are boxed (1–3) and an RGD-like motif is indicated. Other specified amino acids are denoted by the single letter code. Note that amino acid positions are numbered in vertical orientation.