Figure 3

(A). Protein maximum likelihood tree of subfamily 1 nitrilases. The tree was arbitrarily rooted with sequences from the two most closely related subfamilies 2 and 3. Numbers at nodes represent bootstrap support (not shown if <50). (B). Diagram of the gene clusters that include the nitrilase ORF. For environmental genes, the information was limited by the size of the genomic insert. (C). Histogram representing enzymatic enantioselectivity (R or S) on hydroxyglutaronitrile, based on data from [9](na, not assayed; x, not active).