Figure 4From: Functional characterization of two melanocortin (MC) receptors in lamprey showing orthology to the MC1 and MC4 receptor subtypesSaturation curves with Scatchard plot and competition curves for the lamprey MCa receptors expressed in HEK-293 EBNA cells. The saturation curves (left) were obtained with 125I-labelled NDP-MSH and the figure shows total binding (filled square) and binding in the presence of 2 μM cold NDP-MSH (filled triangle). The lines represent the computer-modelled best fit of the data assuming that ligands bound to one site. The competition curves (right) for NDP-MSH (filled triangle, pointing up), α-MSH (filled square), β-MSH (open circle), γ1-MSH (open diamond), ACTH(1–17) (open square), ACTH(1–24) (filled circle), ACTH(1–39) (x), MTII (filled diamond), HS024 (open triangle, pointing down), MSH-A (filled triangle, pointing down), MSH-B (open triangle, pointing up) and ACTH (1–31) (asterisk) were obtained by using a fixed concentration of approx. 0.6 nM 125I-labelled NDP-MSH and varying concentrations of the non-labelled competing peptide.Back to article page