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Table 3 Sites under positive selection on the primate ANG gene

From: Structural and functional implications of positive selection at the primate angiogenin gene

Sites

PAML ω/PP (BEB)

 

TreeSAAP properties

Codo n

Amino acid

M2a

M8

Total

Chemical

Structural

Other

28

4 Ser

3.376 ± 0.53 0.999

3.210 ± 0.518 1.000

9

4

R F , H nc , E sm , R a

5

F , P c , B I , α n , P

0

----

31

7 Thr

3.246 ± 0.735 0.946

3.158 ± 0.610 0.980

4

3

R F , pK', H

1

α n

0

----

32

8 His

3.245 ± 0.73 0.943

3.166 ± 0.590 0.982

1

0

---------

1

α c

0

----

35

11 Thr

3.369 ± 0.547 0.996

3.208 ± 0.521 0.999

3

0

---------

3

Pβ, K0, α c

0

----

56

32 Arg

3.246 ± 0.73 0.943

3.157 ± 0.610 0.979

5

2

pH i , p

3

F, α c , P

0

----

58

34 Gly

3.229 ± 0.740 0.936

3.160 ± 0.607 0.979

9

7

B r , R F , E l , H nc , R a , H p , E t

2

N s , P β

0

----

65

41 Asp

3.263 ± 0.709 0.950

3.173±0.587 0.984

7

3

pK' , P r, p

3

K 0 , F , α c

1

M w

75

51 Arg

3.349 ± 0.584 0.986

3.202 ± 0.534 0.996

5

2

pH i , H nc

3

K0,α c , P

0

----

76

52 Ser*

3.247 ± 0.729 0.946

3.166 ± 0.598 0.983

0

0

---------

0

--------

0

----

87

63 Asn

3.335±0.607 0.980

3.199 ± 0.540 0.995

9

4

B r , μ, P r , p

4

N s , α n , B I , V 0

1

M w

90

66 Arg

3.318 ± 0.635 0.974

3.191 ± 0.555 0.992

16

9

B r , R F , pK' , pH i , E I , R a , H p , H t , E t

7

F , M v , α c , N s , P β , B I , P

0

----

106

82 Lys

3.287 ± 0.680 0.960

3.178 ± 0.579 0.987

4

3

B r , pH i , E t

1

N s

0

----

108

84 His

3.368 ± 0.550 0.995

3.207 ± 0.524 0.998

6

3

pH i , H nc , p

3

F , α c , P

0

----

117

93 Gln

3.367 ± 0.551 0.995

3.208 ± 0.523 0.999

5

3

B r , pH i , E t

2

B I , α c

0

----

127

103Val

3.229 ± 0.75 0.936

3.150 ± 0.628 0.976

1

0

---------

1

α n

0

----

  1. ω and Bayesian (BEB) analysis posterior probabilities obtained with the NJ topology are shown for sites with PP > 0.95 in M8 that also have a PP > 0.90 in M2a. TreeSAAP analysis results present the total number of radical changes in amino acid properties and their assigned categories. Type I sites are shown in bold. *Site 52 had only strong support when using the NJ topology.
  2. Properties symbols are as following: α c : Power to be – C-term., α-helix; α n : Power to be in the N-terminal of an α-helix; B r : Buriedness; B I : Bulkiness; E I : Long-range non-bonded energy; E sm : Short and medium range non-bonded energy; E t : Total non-bonding Energy; F : Mean r.m.s. fluctuation displacement; H : Hydropathy; H nc : Normal consensus hydrophobicity; H p : Surrounding hydrophobicity; H t : Thermodynamic transfer hydrophobicity; K0: Compressibility; μ : Refractive index; M v : Molecular volume; M w : Molecular weight; N s : Average number of surrounding residues; Pα: α- helical tendencies; Pβ: β-structure tendencies; P c : Coil tendencies; P : Turn tendencies; p : Polarity; pH i : Isoelectric point; pK' : Equilibrium Constant of ionization for COOH; P r : Polar requirement; R a : Solvent accessible reduction ratio; R F : Chromatographic index; V0: Partial specific volume;