Phylogenetic analysis of the bHLHs from Amphimedon queenslandica. The represented tree is a NJ tree whose rooting should be considered as arbitrary. This tree is based on a multiple alignment that includes all the Amphimedon queenslandica bHLH (named Amq1 to Amq16) sequences (in blue) and one representative sequence (from Homo sapiens) for each of the bilaterian families of orthologs (in orange). Similar relationships (with similar statistical supports) are found when we used the whole set of bHLHs genes from Homo sapiens. Numbers above the internal branches are as in Figure 2. We only show the statistical support for the internal branches that correspond to monophyletic groups concerning the Amphimedon queenslandica bHLHs. Higher-order groups (A to F) are shown. The Amphimedon queenslandica bHLHs that can be assigned to a family are in grey filled boxes (light grey denotes cases for which there is an uncertainty). Amphimedon queenslandica bHLHs that are associated with more than one family (and the concerned families) are in open black boxes. The single 'orphan' Amphimedon queenslandica bHLH is underlined.