PCR identification of ENVV. A) ENVV2 PCR. Lane 1: Human. Lane 2: B. orangutan. Lane 3: Africa green monkey. Lane 4: Squirrel monkey. Lane 5: Ring-tailed lemur. B) Primate phylogeny, with hypothetical integrations indicated. Below each species is indicated whether a given gene/ORF was detected (+) or not (-). Notes: 1) Contains a 60 amino acid C-terminal truncation. 2) Detected by an internal ENVV PCR (see methods). 3) Not PCR detected.