In vitro protein sintesis using the RTS system. a) and b) The proteins obtained, using complete sequences of env as templates had the sizes of normal Env protein (54 kDa). a) lane1, D. madeirensis; lane2, D. obscura; lane3, D. ambigua; and b) lane1, D. bifasciata; lane2, D. affinis; lane3, S. elmoi. c) The proteins obtained using disrupted sequences of env as templates were truncated (15 kDa). c) lane1, D. pseudoobscura; lane2, D. persimilis; lane3, D. miranda.