Figure 2

KCNE1 proteins from chimpanzee and human are highly N-glycosylated. A HEK293 cells were transfected with 1 μg (lanes 1, 3, and 5) or 3 μg (lanes 2, 4, and 6) expression plasmids for the indicated KCNE1 variants. Nascent (n) proteins and mature (m) proteins were detected by Western blotting. B HEK293 cells transfected with 1 μg expression plasmids were treated without (lanes 1, 3, and 5) and with tunicamycin (lanes 2, 4, and 6). Addition of the N-glycosylation inhibitor tunicamycin results in loss of the glycosylated KCNE1 variants. A, B 15 μg total protein were loaded per lane.