Figure 4

Alignment of the deduced amino acid relaxin sequences from mammals and teleost species used for the phylogenetic analysis. Conserved residues are boxed. Location of the relaxin receptor binding motif residues (RXXXRXXI/V), B-chain, A-chain, and twin dibasic junctions (B/C and C/A) are shown. Amino acids that are underlined are those identified as potential candidates of codon-specific positive selection using the branch-site model A analyses (see text for details), but only the two that are in bold and underlined were found to have a significant probability of being subject to positive selection with a BEB probability >0.95.