Figure 7

Phylogenetic reconstruction of vertebrate gene families with members in chromosomal proximity to both akirin1 and akirin2. The corresponding amino acid alignments employed are provided in additional file 3. The shown topologies (a-g) were obtained by NJ and unless mentioned otherwise in the main text, were comparable to those produced by Bayesian, ML and MP analyses. Branch confidence values greater than 50% from each approach are shown in the order NJ/Bayesian/ML/MP. The ras-related GTP-binding family (rragc and rragd) (a), heterogeneous nuclear ribonucleoprotein family (hnrnp-r and hnrnp-q [HGNC name-Syncrip]) (b), cytosolic 5'-nucleotidase 1 family (nt5c1a and nt5c1b) (c), proline-rich nuclear receptor coactivator family (pnrc1 and pnrc2) (d), cannabinoid receptor family (cnr1 and cnr2) (e) and glycoprotein endo-alpha-1,2-mannosidase family (manea and maneal) (f) each contained two members in all non-teleost vertebrate genomes examined and were, with limited exceptions, present in syntenic chromosomal regions (or double conserved syntenic regions in some teleosts- see fig. 5 and fig. 6) where one family member was located near akirin1 and the other near akirin2. The potassium voltage-gated channel (kcnq) family (Kcnq1/2/3/4/5) (g) contains two family members (kcnq4 and kcnq5) that are, in most vertebrates, in genomic proximity to akirin1 and akirin2 respectively. Further details about each phylogenetic analysis are provided in the text. To summarise, the branching patterns for each of these families was compatible with a duplication event in the vertebrate lineage of chordates and was also concomitant to their genomic location in relation to akirin1 (indicated by red branches) and akirin2 (indicated by blue branches).