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Table 3 PCR primer sequences and priming sites

From: Glutamine synthetase sequence evolution in the mycobacteria and their use as molecular markers for Actinobacteriaspeciation

Name Sequence (5'-3') Product size: Pair Tm (°C) Genome Coordinates
glnA Up F AGATGGACACGGTGGAGT 796 bp 55 2486860
glnA Up R CTTTACTGTATCCGCGGC    2487605
AI FI CACGGTCAGTAACGTCTGC 550 bp 55 2487524
AI RI TCCACCTCGTAGAAGGAGC    2488081
AI FII TTCGATTCGGTGAGCTTC 574 bp 57 2488029
AI RII GCCGCTTGTAGGAGTTCA    2488602
AI FIII ACGACGAGACGGGTTATG 294 bp 54 2488483
AI RIII ATCAGCATGGCCGAGAAC    2488768
AI FIV TGGTCTATAGCCAGCgcA 597 bp 56 2488633
AI RIV GAGATGATTGCCAAGCGG    2489229
  1. Polymerase chain reaction primers used to amplify the glnA1-locus of M. tuberculosis, including its' 5'- and 3' surrounding regions, as overlapping PCR fragments, which facilitated the assembly of the full target region for sequencing (2369 bp).