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Table 1 Genetic variation in markers used for phylogeny reconstruction ( CO1 and ITS1 ) and population genetic analyses

From: Strong genetic structure corresponds to small-scale geographic breaks in the Australian alpine grasshopper Kosciuscola tristis

  CO1 ITS1 Microsatellites (five shared loci) Microsatellites (all typed loci)
Location Nucleotide diversity (π) Number of haplotypes Number of haplotypes H O H E N a H O H E N a
Kosciuszko region          
Mt Jagungal 0.001 2 1 0.61 0.83 11.80 0.56 0.83 11.63
Mt Tate 0.002 4 1 0.51 0.81 12.40 0.51 0.82 11.88
Guthega 0.002 4 3 0.50 0.81 13.60 0.48 0.82 12.00
Mt Townsend 0.001 3 1       
Thredbo 1 0.001 2 1 0.56 0.82 12.40 0.53 0.79 10.25
Thredbo 2     0.51 0.83 13.20 0.48 0.80 11.25
Thredbo 3     0.57 0.81 13.80 0.50 0.77 11.25
Victoria          
Mt Bogong 0.008 2 1 0.46 0.88 13.40    
Mt Nelse 0.001 4 2       
Falls Creek 0.000 1 1 0.51 0.90 14.20    
Mt Cope 0.000 1 1 0.38 0.87 12.20    
Mt Jaithmathang 0.002 2 1       
Mt Hotham 0.041 5 2       
Mt Buffalo 0.002 5 1 0.26 0.80 9.80    
Mt Buller 0.001 2 1 0.49 0.78 8.80    
Mt Stirling 0.000 1 1 0.33 0.69 10.00    
Mt Baw Baw 0.002 3 5 0.27 0.59 7.40    
  1. Genetic variation was measured as nucleotide diversity and haplotype number for CO1; nucleotide diversity was not calculated for ITS1 as variation was very low. Observed heterozygosity (Ho), expected heterozygosity (HE) and mean allelic richness (Na) were calculated for five microsatellite loci that amplified across all populations, as well as for eight loci typed for the Kosciuszko populations.