Population structure of a microparasite infecting Daphnia: spatio-temporal dynamics

Table 1 Summary of analysed Caullerya mesnili samples from seven lakes in the Czech Republic

DNA	Lake	Year	TCS-type										No. of ITS
Extraction			C1	C2	C3	C4	C5	C6	C7	C8	C9	C10	Sequences
20 pooled hosts/lake sample ^a
	Brno	2004	6	0	0	10	0	11	0	0	0	0	27
	Brno	2005	2	0	0	12	1	2	0	14	0	1	32
	Římov^c	2004	3	0	0	19	0	7	0	1	0	0	30
	Římov	2005	2	0	0	22	0	4	0	0	1	0	29
	Římov	2008	0	0	0	15	0	4	0	10	0	0	29
	Římov	2009	3	0	0	15	0	8	0	4	0	0	30
	Stanovice	2004	3	0	0	18	0	5	0	1	0	0	27
	Stanovice	2005	3	0	0	11	1	10	0	0	0	0	25
	Trnávka	2005	8	0	0	12	0	7	0	0	0	0	27
	Vír^c	2004	9	0	0	2	0	17	0	0	0	0	28
	Vír	2005	3	0	0	15	0	6	0	1	0	0	25
	Vír	2009	4	0	0	5	0	17	0	0	0	0	26
	Vranov	2004	2	0	0	10	0	14	0	3	0	0	29
	Vranov	2008	3	0	0	22	0	7	0	0	0	0	32
	Vranov	2009	3	0	0	12	0	18	0	0	0	0	33
	Želivka^c	2004	1	0	0	8	2	6	0	11	0	0	28
		∑	55	0	0	208	4	143	0	45	1	1	457
10 single hosts/lake sample ^b
	Římov^c	2004	19	3	0	66	0	20	0	9	0	0	117
	Vír^c	2004	34	1	1	20	0	56	0	0	0	0	112
	Želivka^c	2004	10	0	0	26	7	22	1	43	0	0	109
		∑	63	4	1	112	7	98	1	52	0	0	338

Specifically, the number of ITS sequences obtained from C. mesnili parasite DNA, as well as their assignment to representative sequence variants (TCS-types) by statistical parsimony analysis (C1-C10, the labels are consistent with [38]) are provided. Parasite DNA was either extracted from 10 individual Daphnia hosts per sample or 20 pooled Daphnia hosts per sample. Table modified after Giessler and Wolinska [40].
^aParasite data from Giessler and Wolinska [40].
^bParasite data from Wolinska et al. [38].
^cParasite DNA from these samples was analysed using both approaches (i.e. 10 single hosts and 20 pooled hosts were genotyped, respectively).

ISSN: 2730-7182