Fig. 3

Spatial analysis of gene expression after Notch signaling perturbation. Hnf1 (a, b), Ptf1a (c, d), Mist1 (e, f) and Fng (g, h) transcript localization tested by FISH in control animals (a, c, e, g) and in animals treated with DAPT (b, d, f, h). In all the experiments, Ptf1a (in green) was used as second probe in double FISH (in a, b, e, f, g, h) since the increase of Ptf1a + cells in treated larvae confirms the phenotype. Red arrow in D indicates Ptf1 ectopic expression in a few cells of the anus. Yellow circles in E show that Mist1 is expressed in alternated cells of the apical organ in control larvae, while arrows in F indicate that in treated larvae Mist1 is expressed in adjacent cells of the apical organ (yellow arrow) and in a few cells of the esophagus (white arrow). For each analyzed gene, quantification of the phenotypes is shown on the right. Abbreviations: AO, apical organ; es, esophagus; lv, lateral view; MG, midgut; st, stomach, v, ventral