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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Subfunctionalization of peroxisome proliferator response elements accounts for retention of duplicated fabp1 genes in zebrafish

Fig. 2

The PPAR-dependent induction of fabp1a and fabp1b.1 mRNA was tissue-specific. Explant liver and intestine cells were cultured for 48 h before being treated with 1 μM WY14643 (PPARα-specific agonist) or rosiglitazone (PPARγ-specific agonist) for 24 h. fabp1a, fabp1b.1, and fabp1b.2 mRNA levels were quantified by qRT-PCR using the ΔΔCT method and normalized to GAPDH. Data are mean ± SEM. *P < 0.001 compared to vehicle treatment within tissue and transcript, †P < 0.001 compared to WY14643 treatment within tissue and transcript as determined by two-way ANOVA followed by Bonferroni’s post-hoc test. n = 4

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