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Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Subfunctionalization of peroxisome proliferator response elements accounts for retention of duplicated fabp1 genes in zebrafish

Fig. 5

PPRE mutagenesis identified sites for PPARα- and PPARγ-selective induction of zebrafish fabp1a and fabp1b.1 promoter activity, respectively. Site-directed mutagenesis was used to alter the 5′ flanking region (Δ5′FR) or direct repeat element (ΔDR1) PPAR binding sites in the fabp1a (a) and fabp1b.1 (b) promoter fragments. Firefly luciferase activity driven by the fabp1a (c, d) or fabp1b.1 (e, f) promoters was normalized to Renilla luciferase activity driven by the TK promoter in HEK293A cells treated with 1 nM – 1 mM WY14643 (PPARα agonist) (c, e) or rosiglitazone (PPARγ agonist) (d, f) for 24 h. Data are mean ± SD. *P < 0.001 compared to fabp1a/TK or fabp1b.1/TK alone, ^P < 0.001 compared to fabp1a Δ5′FR /TK or fabp1b.1 Δ5′FR /TK within agonist dose as determined via two-way ANOVA followed by Bonferroni’s post-hoc test. n = 3

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