Fig. 4
The abundance of the CenSol repeats in fire ant genomes. a Detection of the CenSol repeat by restriction digestion of S. invicta and S. geminata genomic DNA. Equal amounts of uncut or restriction digested (EcoRV or BsaAI) genomic DNA were separated by agarose gel electrophoresis and then stained with SYBR Safe (left panel). Southern analysis of the DNA from the left panel transferred to a nylon membrane with the CenSol probe (right panel). Two types of ladder-like patterns (arrowheads, main repeat; arrows, ‘off’ ladder repeats due to a BsaAI polymorphism) are indicated. b Slot blot hybridization to estimate the relative amounts of the CenSol sequence between the S. invicta and S. geminata genomes. DNA amounts are labeled