Fig. 3From: Evolution of cytokinesis-related protein localization during the emergence of multicellularity in volvocine green algaeImmunofluorescence images of DRP1 localization in Chlamydomonas reinhardtii. Differential interference contrast (DIC) images (a, e, i), immunofluorescence images labeled with an anti-TsDRP1 (b, f, j) and an anti-tubulin alpha (c, g, k), and merged immunofluorescence images of DRP1 and tubulin (d, h, l) are shown. In vegetative cells, DRP1 was localized to cytoplasm (b-d). DRP1 was localized to a first division plane of two-celled embryo (white arrowheads in f-h) and second division planes of four-celled embryo (black arrowheads in j-l) mainly. Note that DRP1 signals localized to the first division plane of the two-celled embryo (f) become weak in the four-celled stage (j). The first and second division planes in the four-celled stage were estimated by relative positions of pyrenoids. Scale bars: 5 μmBack to article page