Double maternal-effect: duplicated nucleoplasmin 2 genes, npm2a and npm2b, with essential but distinct functions are shared by fish and tetrapods

Table 1 Defects in F1 embryos derived from crossing wild type males to F0 females that had been injected with npm2a or npm2b CRISPR mRNA as embryos

Penetrance of npm2a and npm2b mutant phenotypes
	Total number of embryos	Embryos with defects
	Total number of embryos	Non-cellularized^†	Partially cellularized^‡	Normal embryos	% Abnormal embryos
npm2a-1	741	366	0	375	49%
npm2a-2	252	237	0	15	94%
npm2a-3	107	82	0	25	77%
npm2a-4	150	147	0	3	98%
npm2b-1	105	47	42	16	85%
npm2b-2	66	60	6	0	100%
npm2b-3	263	129	134	0	100%
npm2b-4	525	347	173	5	99%
npm2b-5	376	100	92	184	51%

npm2a nucleoplasmin 2a, npm2b nucleoplasmin 2b
This table demonstrates representative data from a single clutch from each mutant female. ^†Embryos did not develop at all (please refer to Fig. 7e-l). ^‡Embryos had a partially cellularized blastodisc that was sitting atop an enlarged yolk syncytial layer (please refer to arrows in Fig. 7n and o)

ISSN: 2730-7182