Fig. 5From: Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strengthAltered gene expression in evolved strains is focused on transport pathways. Genes were considered to be significantly changes with a Bayes p-value score of less than 0.05 and a pfp value of less than 0.05. Genes found to change significantly in the evolved strains were grouped into functional classes. The p-value is the summation of the hypergeometrical distribution and p-values less than 0.05 were considered to be significant. a Over-represented KEGG BRITE classes found. b Over-represented regulons found. c The expression of genes involved in glucose uptake in L. lactis MG1363 were significantly changed in the evolved strains. Highlighted in green are genes that were up-regulated and in red for down-regulated. Gene expression changes not meeting the significance cut-off values are highlighted in grey. d Kinetic parameters of glucose transport in Genr0 and the evolved stains were determined in cells grown to exponential phase in CDMPC. Glucose transport was assayed with the use of [14-C]-labeled glucose. Values of three independent experiments were averaged and are reported ±SD. Vmax and Km were determined using glucose concentrations from 1.2 to 200 μMBack to article page