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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Are there gap junctions without connexins or pannexins?

Fig. 3

Electrical coupling in A. rubens early embryos. a-c Microelectrode V1 placed in one blastomere registers the membrane potential changes, whereas I2 electrode inside the adjacent blastomere is used to inject current pulses. a, b Fluorescent dye applied via V1 electrode stain only injected cell . Plain light background a and fluorescence image b of 2-cell embryo. c Negative current impulses (red) are applied to test electrical coupling. Electrical potential changes (blue) are recorded via V1 electrode. (a, b and c are from the same experiment). d. V1/I2 values in two different situations from 51 current impulse experiments. The difference between IC and GJ is significant (p-value<‚ÄČ0.05). On the left (A) V1/I2 value was measured in incompletely cleaved zygote and corresponds to cell input resistance R, on the right (B) V1/I2 was measured in separated blastomeres

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