Table 3 Identification of unique miR394 sequences and their predicted targets using psRNATarget server. List of Unique miR394s (UmiR394s), their Unique Target Sequences (UTSs) which were complementary binding site of UmiR394s bearing a unique identification number (UR). The UTSs were used for the target identification through psRNATarget server. The targets were selected on the basis of E-Value and unpaired energy (UPE) evaluated on the basis of target-site accessibility and mode of inhibition

1

ath-miR394a,ath-miR394b-5p,osa-miR394,sbi-miR394a,b,zma-miR394a-5p,zma-miR394b-5p,ptc-miR394a-5p,ptc-miR394b-5p,vvi-miR394b,ghr-miR394a,b,aly-miR394a-5p,aly-miR394b-5p,ahy-miR394,gma-miR394b-5p,gma-miR394a-5p,gma-miR394c-5p,d,e,f,g,csi-miR394,tcc-miR394a,b,bdi-miR394,ssl-miR394,bna-miR394a,b,mes-miR394a,b,cca-miR394,lus-miR394a,b,nta-miR394,ppe-miR394a,b,mdm-miR394a,b,cme-miR394a,b,cpa-miR394a,b,atr-miR394,sly-miR394–5p,ata-miR394–5p,ini-miR394,csa-miR394a,b,c,nnu-miR394,jre-miR394,mac-miR394,era-miR394,nto-miR394,rsa-miR394a,b,c,aip-miR394,adu-miR394,egu-miR394 cst-miR394a,b,fve-miR394,peu-miR394a,b

UUGGCAUUCUGUCCACCUCC

1a

GGAGGUUGACAGAAUGCCAA

1

14.87

AT1G27340

Ath-LCR, LEAF CURLING RESPONSIVENESS

Cleavage

1

16.78

POPTR_0001s13770g

Ptc-F-box family protein

Cleavage

1

17.38

POPTR_0003s16980g

Ptc-hypo (F-box)

Cleavage

1

16.82

LOC100242085

Vvi-F-box family protein

Cleavage

1

15.82

LOC100800464

Gma-F-box only protein 6-like (1)

Cleavage

1

13.67

LOC100810179

Gma-F-box only protein 6-like (2)

Cleavage

1

15.65

LOC100795488

Gma-F-box only protein 6-like (3)

Cleavage

1

16.47

LOC109152509

Ini-F-box only protein 6-like (1)

Cleavage

1

14.044

LOC109175636

Ini-F-box only protein 6-like (2)

Cleavage

1

14.867

LOC104757055

Csa-F-box only protein 6-like (1)

Cleavage

1

15.009

LOC104741342

Csa-F-box only protein 6-like (2)

Cleavage

1

14.867

LOC104776781

Csa-F-box only protein 6-like (3)

Cleavage

1

17.161

LOC104592877

Nnu-F-box only protein 6-like (1)

Cleavage

1

16.572

LOC104591738

Nnu-F-box only protein 6-like (2)

Cleavage

1

15.37

LOC109003194

Jre-F-box only protein 6-like (1)

Cleavage

1

16.4

LOC104415679

Era-F-box only protein 6

Cleavage

1

17.6

LOC104116922

Nto-F-box only protein 6

Cleavage

1

17.61

LOC108809705

Rsa-F-box only protein 6

Cleavage

1

17.1

LOC101211089

Cst-F-box only protein 6

Cleavage

1

15.55

LOC101312115

Fve-F-box only protein 6

Cleavage

1b

GGAUGUGUGCAGAGUGCCAA

3

15.92

AT3G48460

Ath-GDSL-motif esterase/acyltransferase/lipase

Cleavage

1c

GGAGGAGGACAGAGAUGCCAA

3

24.57

AT5G09670

Ath-loricrin-related

Cleavage

1d

GGAGGUGGAAGAAUGCCGG

3

20.99

AT3G12120

Ath-ATFAD2, FAD2, FATTY ACID DESATURASE 2

Translation

1e

GGAGGUGGACAGAAUGCCAA

0

21.42

Os01 g69940

Osa-OsFBX32 - F-box domain containing protein

0

23.55

SORBIDRAFT_03g044270

Sbi-hypo 1 (F-box domain)

Cleavage

0

23.11

LOC103636344

Zma-F-box only protein 6-like (not annotated)

Cleavage

0

22.96

LOC100193727

Zma-Uncharacterized1 (F-box only protein 6-like)

Cleavage

0

22.083

LOC103972833

Mac-F-box only protein 6

Cleavage

1f

GGAGGUCGACAGAAUGCCAA

1

14.89

LOC105962383

Egu-F-box only protein 6

Cleavage

1 g

GGAGUUGGACAGAAUGCAAA

2.5

13.3

Os05g51150

Osa-RNA polymerase sigma factor

Cleavage

1 h

CAAGGUGGACAGAAUGCUAA

2.5

16.14

SORBIDRAFT_02g034550

Sbi-hypo 2 (Transcription factor GRAS)

Cleavage

1i

GGAAGUGGACAGAGUGCUGA

2.5

18.13

LOC100242751

Vvi-PMT18

Cleavage

1j

GAAGGUGGACAGAGUGCUAC

3

20.26

LOC100253052

Vvi-FGGY carbohydrate kinase domain-containing protein

Cleavage

1 k

GGAGGUAGACAGAAUGCCAA

1

18.77

LOC109010064

Jre-F-box only protein 6-like (2)

Cleavage

1

14.65

LOC107631986

Aip-F-box only protein 6

Cleavage

1

14.65

LOC107482128

Adu-F-box only protein 6

Cleavage

1

15.63

LOC105133704

Peu-F-box only protein 6-like (1)

Cleavage

1

16.33

LOC105123018

Peu-F-box only protein 6-like (2)

Cleavage

2

vvi-miR394a,c,mes-miR394c

UUGGCAUUCUGUCCACCUCCAU

2

AAGGAGGUUGACAGAAUGCCAA

1

16.82

LOC100242085

Vvi-F-box family protein

Cleavage

3

ptc-miR394a-3p,ptc-miR394b-3p

CUGUUGGUCUCUCUUUGUAA

3

UUACAAAGAGAGACCAACAG

0

17.4

POPTR_0002s11320g

Ptc-Hypo1 (DUF1005)

Cleavage

4

gma-miR394a-3p

AGCUCUGUUGGCUACACUUU

4

UGAGUGCAGCCAGCAGAGCU

3

14.74

LOC100306608

Gma-Unch (DUF3774)

Cleavage

5

zma-miR394a-3p,zma-miR394b-3p,ata-miR394–3p

AGGUGGGCAUACUGCCAAUG

5

CAUUGGCAGUAUGCCCACCU

0

27.31

LOC100383214

Zma-Uncharacterized 2

Cleavage

6

sly-miR394–3p,gma-miR394b-3p

AGGUGGGCAUACUGUCAAC

6

NA

–

–

–

–

–

7

ath-miR394b-3p,aly-miR394a-3p

AGGUGGG[C/U]AUACUGCCAAUA

7

NA

–

–

–

–

–

8

aly-miR394b-3p

AGGUGGACAUAUUGCCAACA

8

CGUUGGUAAUAUGUCUGCCU

2.5

20.27

XM_002894202

Aly-Hypo (Pectinesterase inhibitor domain)

Cleavage