Fig. 3

Evolution of expression in TF WGD-duplicates. a An illustration of how the z-scores in b are calculated. Individual TF duplicates were assigned to a cell using the extant (x-axis) and ancestral (y-axis) expression quartile values (dark green = 4th, green = 3rd, yellow = 2nd, white = 1st). Z-scores were then determined by comparing the frequency of the observed values to frequency distribution that would be expected if expression values were chosen randomly from a pool of extant and ancestral values. b Difference in expression quartile of individual TFs compared to their ancestors. Heatmaps show the z-scores of the observed frequency of each difference compared to the expected frequency for LightDev (left column) and Diff (right column) dataset in three WGD events (α = top, β = middle, γ = bottom). Darker red and blue indicates counts higher and lower than random expectation, respectively. c An illustration of how the z-scores in d were calculated. For each WGD TF duplicate, the difference in the expression quartile values (colored the same as in (a)) of an extant duplicate and its ancestral gene is defined as “deviation”. Duplicate 1 is the copy with a higher or equal expression quartile value compared to the other copy (duplicate 2). d Deviation values of pairs of TF WGD-duplicates. Heatmaps show the z-scores of the observed frequency of WGD-duplicate pair deviation compared to the expected frequency for LightDev (left column) and Diff (right column) datasets in three WGD events (α = top, β = middle, γ = bottom). Color correlates with the magnitude of the z-score as in (a)