Singleton molecular species delimitation based on COI-5P barcode sequences revealed high cryptic/undescribed diversity for Chinese katydids (Orthoptera: Tettigoniidae)

Table 4 MOTU number of Chinese Katydids inferred by each single-locus species delimitation methods

Quantity	DBCHL	DBMEC	DBPPM	DBTB	Total
COI-5P sequences	596	376	993	200	2165
Haplotypes	390	158	530	147	1225
Identified morphospecies/BINs/Record count	40/69/579	40/51/341	43/63/551	8/13/164	131/196/1635
Singleton BINs/Record count	11/11	20/20	19/19	2/2	52/52
Concordant BINs/Record count	55/502	27/288	44/532	10/54	136/1376
Discordant BINs/Record count	3/66	4/33	0/0	1/108	8/207
Unidentified BIN-species/Record count	8/17	5/35	118/441	19/36	150/529
Singleton BINs/Record count	2/2	4/4	57/57	10/10	73/73
Concordant BINs/Record count	6/15	1/31	61/384	9/26	77/456
Total BINs count/Record count	77/596	56/376	181/992^a	32/200	346/2164
RESL^*	71	56	180	42	349
jMOTU (13 bp)	61	57	169	31	318
ABGD (2.15%)	42	53	140	20	255
Single-GMYC	87	66	197	32	382
Multiple-GMYC	94	60	206	37	397
bPTP	55	56	178	23	312

Record count, the number of COI-5P sequences after quality control, ^*Alignment: MUSCLE (Edgar, 2004). Filters applied: ≥ 600 bp only, exclude records contained contaminants, had stop codons, flagged as misidentifications or errors; Deletion method: Pairwise deletion; One COI-5P sequence without BIN records in DBPPM dataset corresponded that sequences did not fulfill with barcode compliance standards. All bPTP results were from Bayesian MCMC analyses. Results of GMYC and bPTP analyses were from genealogies based on COI-5P haplotype sequences

ISSN: 2730-7182