Fig. 8
The numbers of genetic changes acquired by clones after 700 generations of evolution at pH 7.5 (weak nitrite [NO2−] toxicity, weak interdependence) sorted by the functional annotations of the target genes. The horizontal bar and P-value indicates a statistically significant outcome from a two-sample Wilcoxon rank-sum test. The star indicates a P-value less than 0.05. Data are presented as Tukey box plots. The numbers of genetic changes include synonymous and non-synonymous point mutations, insertions, deletions, and multiplications. The nitrite cross-feeding clones acquired genetic changes that were unique to nitrite cross-feeding. Functional categories (COG): S, function unknown; N, cell motility; T, signal transduction; K, transcription; L, replication, recombination and repair; O, posttranslational modification, protein turnover and chaperones; Q, secondary metabolites biosynthesis, transport and catabolism; J, translation, ribosomal structure and biogenesis; M, cell wall/membrane/envelope biogenesis